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   set  data   graph     files  public [IODP360 - CHNS analyses] - Supplementary Table 1: Results of CHNS analyses in rock samples
at different depths and sample images. (Collaborative Research: Delineating The Microbial
Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-
omics and Culturing Approaches)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_810902

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Powderized rock material 462 from each sample (produced in the laminar flow
hood on the JOIDES Resolution using a sterile mortar and pestle) was
immediately transferred to sterile, muffled glass containers and stored in a
desiccator until analysis of carbon and nitrogen according to established
methods. Briefly, samples were weighed into methanol rinsed silver boats (4 x
6 mm, Costech). 96-well glass plates (combusted 4 hrs at 450\u00b0C) holding
these samples were placed in a vacuum desiccator that also contained an open
dish with about 50 mL fresh, concentrated (12N) HCl. An inverted
crystallization dish was placed over the samplesto protect them from water
condensation. The desiccator was closed and pumped out with an airdriven
aspirator, to a reading of about ~ 0.5 atm and the desiccator was placed in an
oven kept between 60 and 65 \u00b0C. Acidification was allowed to run for 60
to 72 hours. When acidification was complete, the samples were removed and set
in the oven to dry (60-65 \u00b0C), and then placed in a vacuum desiccator
charged with indicating silica gel (Fisher S162-500, activated by heating
above 220 \u00b0C for several hours) and pumped down again and dried for about
24 hours.

\u00a0
attribute NC_GLOBAL awards_0_award_nid String 709555
attribute NC_GLOBAL awards_0_award_number String OCE-1658031
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1658031 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String SUpplementary Table 1: CHNS analysis
PI: Virginia Edgcomb
Data Version 1: 2020-05-11
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL dataset_current_state String Final and no updates
attribute NC_GLOBAL date_created String 2020-05-08T12:26:32Z
attribute NC_GLOBAL date_modified String 2020-07-08T16:51:07Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.810902.1
attribute NC_GLOBAL Easternmost_Easting double 57.278183
attribute NC_GLOBAL geospatial_lat_max double -32.70567
attribute NC_GLOBAL geospatial_lat_min double -32.70567
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double 57.278183
attribute NC_GLOBAL geospatial_lon_min double 57.278183
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 747.7
attribute NC_GLOBAL geospatial_vertical_min double 10.7
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/810902 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String IR Mass Spec
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 810915
attribute NC_GLOBAL instruments_0_description String The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB16/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Isotope-ratio Mass Spectrometer
attribute NC_GLOBAL instruments_0_instrument_nid String 469
attribute NC_GLOBAL instruments_0_supplied_name String DeltaPlus isotope ratio mass spectrometer
attribute NC_GLOBAL instruments_1_acronym String Costech ECS 4010
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 810914
attribute NC_GLOBAL instruments_1_description String The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.
attribute NC_GLOBAL instruments_1_instrument_name String Costech International Elemental Combustion System (ECS) 4010
attribute NC_GLOBAL instruments_1_instrument_nid String 793023
attribute NC_GLOBAL instruments_1_supplied_name String Costech 4010 Elemental Analyzer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, clay, Clay_OH, data, dataset, depth, dmo, erddap, latitude, longitude, management, observations, oceanography, office, picture, preliminary, ratio, Ratio_13C_12C, reference, Reference_Picture, sample, Sample_ID, toc, ton, total, Total_C_Total_N
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/810902/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/810902 (external link)
attribute NC_GLOBAL Northernmost_Northing double -32.70567
attribute NC_GLOBAL param_mapping String {'810902': {'Latitude': 'flag - latitude', 'Depth': 'flag - depth', 'Longitude': 'flag - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/810902/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_0_affiliation_acronym String WHOI
attribute NC_GLOBAL people_0_person_name String Virginia P. Edgcomb
attribute NC_GLOBAL people_0_person_nid String 51284
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_1_affiliation_acronym String WHOI
attribute NC_GLOBAL people_1_person_name String Virginia P. Edgcomb
attribute NC_GLOBAL people_1_person_nid String 51284
attribute NC_GLOBAL people_1_role String Contact
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Karen Soenen
attribute NC_GLOBAL people_2_person_nid String 748773
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String Subseafloor Lower Crust Microbiology
attribute NC_GLOBAL projects_0_acronym String Subseafloor Lower Crust Microbiology
attribute NC_GLOBAL projects_0_description String NSF abstract:
The lower ocean crust has remained largely unexplored and represents one of the last frontiers for biological exploration on Earth. Preliminary data indicate an active subsurface biosphere in samples of the lower oceanic crust collected from Atlantis Bank in the SW Indian Ocean as deep as 790 m below the seafloor. Even if life exists in only a fraction of the habitable volume where temperatures permit and fluid flow can deliver carbon and energy sources, an active lower oceanic crust biosphere would have implications for deep carbon budgets and yield insights into microbiota that may have existed on early Earth. This is all of great interest to other research disciplines, educators, and students alike. A K-12 education program will capitalize on groundwork laid by outreach collaborator, A. Martinez, a 7th grade teacher in Eagle Pass, TX, who sailed as outreach expert on Drilling Expedition 360. Martinez works at a Title 1 school with ~98% Hispanic and ~2% Native American students and a high number of English Language Learners and migrants. Annual school visits occur during which the project investigators present hands on-activities introducing students to microbiology, and talks on marine microbiology, the project, and how to pursue science related careers. In addition, monthly Skype meetings with students and PIs update them on project progress. Students travel to the University of Texas Marine Science Institute annually, where they get a campus tour and a 3-hour cruise on the R/V Katy, during which they learn about and help with different oceanographic sampling approaches. The project partially supports two graduate students, a Woods Hole undergraduate summer student, the participation of multiple Texas A+M undergraduate students, and 3 principal investigators at two institutions, including one early career researcher who has not previously received NSF support of his own.
Given the dearth of knowledge of the lower oceanic crust, this project is poised to transform our understanding of life in this vast environment. The project assesses metabolic functions within all three domains of life in this crustal biosphere, with a focus on nutrient cycling and evaluation of connections to other deep marine microbial habitats. The lower ocean crust represents a potentially vast biosphere whose microbial constituents and the biogeochemical cycles they mediate are likely linked to deep ocean processes through faulting and subsurface fluid flow. Atlantis Bank represents a tectonic window that exposes lower oceanic crust directly at the seafloor. This enables seafloor drilling and research on an environment that can transform our understanding of connections between the deep subseafloor biosphere and the rest of the ocean. Preliminary analysis of recovered rocks from Expedition 360 suggests the interaction of seawater with the lower oceanic crust creates varied geochemical conditions capable of supporting diverse microbial life by providing nutrients and chemical energy. This project is the first interdisciplinary investigation of the microbiology of all 3 domains of life in basement samples that combines diversity and "meta-omics" analyses, analysis of nutrient addition experiments, high-throughput culturing and physiological analyses of isolates, including evaluation of their ability to utilize specific carbon sources, Raman spectroscopy, and lipid biomarker analyses. Comparative genomics are used to compare genes and pathways relevant to carbon cycling in these samples to data from published studies of other deep-sea environments. The collected samples present a rare and time-sensitive opportunity to gain detailed insights into microbial life, available carbon and energy sources for this life, and of dispersal of microbiota and connections in biogeochemical processes between the lower oceanic crust and the overlying aphotic water column.
About the study area:
The International Ocean Discovery Program (IODP) Expedition 360 explored the lower crust at Atlantis Bank, a 12 Ma oceanic core complex on the ultraslow-spreading SW Indian Ridge. This oceanic core complex represents a tectonic window that exposes lower oceanic crust and mantle directly at the seafloor, and the expedition provided an unprecedented opportunity to access this habitat in the Indian Ocean.
attribute NC_GLOBAL projects_0_end_date String 2020-01
attribute NC_GLOBAL projects_0_geolocation String SW Indian Ridge, Indian Ocean
attribute NC_GLOBAL projects_0_name String Collaborative Research: Delineating The Microbial Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-omics and Culturing Approaches
attribute NC_GLOBAL projects_0_project_nid String 709556
attribute NC_GLOBAL projects_0_start_date String 2017-02
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double -32.70567
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String latitude,longitude
attribute NC_GLOBAL summary String Results of CHNS analyses in rock samples at different depths and sample images taken on board of the R/V JOIDES Resolution between November 30, 2015 and January 30, 2016
attribute NC_GLOBAL title String [IODP360 - CHNS analyses] - Supplementary Table 1: Results of CHNS analyses in rock samples at different depths and sample images. (Collaborative Research: Delineating The Microbial Diversity and Cross-domain Interactions in The Uncharted Subseafloor Lower Crust Using Meta-omics and Culturing Approaches)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double 57.278183
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.5
variable Sample_ID   String  
attribute Sample_ID bcodmo_name String sample
attribute Sample_ID description String Sample ID
attribute Sample_ID long_name String Sample ID
attribute Sample_ID nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute Sample_ID units String unitless
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 10.7, 747.7
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Depth below sea floor
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable TOC   float  
attribute TOC _FillValue float NaN
attribute TOC actual_range float 0.006, 0.018
attribute TOC bcodmo_name String TOC
attribute TOC description String Total Organic Carbon
attribute TOC long_name String TOC
attribute TOC nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/CORGCOTX/ (external link)
attribute TOC units String percentage (%)
variable TON   String  
attribute TON bcodmo_name String TON
attribute TON description String Total Organic Nitrogen - bd = below detection limit
attribute TON long_name String TON
attribute TON nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/NTOTZZZZ/ (external link)
attribute TON units String percentage (%)
variable Total_C_Total_N   float  
attribute Total_C_Total_N _FillValue float NaN
attribute Total_C_Total_N actual_range float 4.08, 12.4
attribute Total_C_Total_N bcodmo_name String C_to_N
attribute Total_C_Total_N description String Total Carbon/Total Nitrogen
attribute Total_C_Total_N long_name String Total C Total N
attribute Total_C_Total_N units String percentage (%)
variable Ratio_13C_12C   float  
attribute Ratio_13C_12C _FillValue float NaN
attribute Ratio_13C_12C actual_range float -27.8, -23.0
attribute Ratio_13C_12C bcodmo_name String d13C_DIC
attribute Ratio_13C_12C description String Ratio of stable isotopes 13C/12C
attribute Ratio_13C_12C long_name String Ratio 13 C 12 C
attribute Ratio_13C_12C nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/D13CMIBX/ (external link)
attribute Ratio_13C_12C units String percentage (%)
variable Clay_OH   float  
attribute Clay_OH _FillValue float NaN
attribute Clay_OH actual_range float 0.0, 1.083
attribute Clay_OH bcodmo_name String percent composition
attribute Clay_OH description String wt% OH from clays (150-300°C)
attribute Clay_OH long_name String Clay OH
attribute Clay_OH units String percentage (%)
variable Observations   String  
attribute Observations bcodmo_name String sample_descrip
attribute Observations description String Lithology and alteration observations
attribute Observations long_name String Observations
attribute Observations units String unitless
variable Reference_Picture   String  
attribute Reference_Picture bcodmo_name String sample_descrip
attribute Reference_Picture description String Sample image. See "Data Files" section of metadata for .zip file of images.
attribute Reference_Picture fileAccessBaseUrl String https://datadocs.bco-dmo.org/docs/Subseafloor_Lower_Crust_Microbiology/data_docs/810902/pictures/ (external link)
attribute Reference_Picture long_name String Reference Picture
attribute Reference_Picture units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double -32.70567, -32.70567
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude, south is negative
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double 57.278183, 57.278183
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude, west is negative
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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