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     data   graph     files  public [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata
collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr
2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological
diversification in sponges)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.953999.1
attribute NC_GLOBAL Easternmost_Easting double -81.39122
attribute NC_GLOBAL geospatial_lat_max double 24.56779
attribute NC_GLOBAL geospatial_lat_min double 24.56779
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -81.39122
attribute NC_GLOBAL geospatial_lon_min double -81.39122
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/953999 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL Northernmost_Northing double 24.56779
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 24.56779
attribute NC_GLOBAL summary String Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body.

In this dataset, we present the 16S rRNA microbiome NCBI accession and sample collection metadata for an artificial reef experiment where sponges of 10 species were placed on this temporary reef from April to August of 2021 and sampled using VacuSIP. VacuSIP methods capture incurrent (In) and excurrent (Ex) water from each sponge specimen. Incurrent represents the bacteria that are available for the sponge to consume via filter feeding and excurrent represents the bacteria that remain once sponges have consumed their preferred taxa. Additionally, we have provided microbiome metadata for the host sponges for several of these paired In/Ex samples. See the related dataset, NCBI Bioproject PRJNA1179970, for all sequence data. Microbiome data was generated using protocols from the Earth Microbiome project and sequencing was conducted on an Illumina MiSeq at Middle Tennessee State University. The data available at NCBI represents raw sequencing data, and no quality checks or sequencing filtering has been done on the uploaded sequences.
attribute NC_GLOBAL title String [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr 2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges)
attribute NC_GLOBAL Westernmost_Easting double -81.39122
variable collection_date   String  
attribute collection_date long_name String Collection_date
attribute collection_date units String unitless
variable latitude   float  
attribute latitude _CoordinateAxisType String Lat
attribute latitude actual_range float 24.56779, 24.56779
attribute latitude axis String Y
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   float  
attribute longitude _CoordinateAxisType String Lon
attribute longitude actual_range float -81.39122, -81.39122
attribute longitude axis String X
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable accession   String  
attribute accession long_name String Accession
attribute accession units String unitless
variable sample_name   String  
attribute sample_name long_name String Sample_name
attribute sample_name units String unitless
variable species_ab   String  
attribute species_ab long_name String Species_ab
attribute species_ab units String unitless
variable sample_type   String  
attribute sample_type long_name String Sample_type
attribute sample_type units String unitless
variable organism   String  
attribute organism long_name String Organism
attribute organism units String unitless
variable AphiaID   String  
attribute AphiaID long_name String Aphiaid
attribute AphiaID units String unitless
variable LSID   String  
attribute LSID long_name String Lsid
attribute LSID units String unitless
variable isolation_source   String  
attribute isolation_source long_name String Isolation_source
attribute isolation_source units String unitless
variable geo_loc_name   String  
attribute geo_loc_name long_name String Geo_loc_name
attribute geo_loc_name units String unitless
variable data_day   int  
attribute data_day actual_range int 0, 6
attribute data_day long_name String Data_day
attribute data_day units String days
variable seq_attempt   String  
attribute seq_attempt long_name String Seq_attempt
attribute seq_attempt units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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