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     data   graph     files  public [Carb microarray analysis Atlantic 2019] - Carbohydrate microarray (epitope) analyses of POM-
derived carbohydrates in the Western North Atlantic Ocean in May 2019 (A mechanistic microbial
underpinning for the size-reactivity continuum of dissolved organic carbon degradation)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_985786_v1

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.985786.1
attribute NC_GLOBAL Easternmost_Easting double -53.3949
attribute NC_GLOBAL geospatial_lat_max double 42.83954
attribute NC_GLOBAL geospatial_lat_min double 34.50102
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -53.3949
attribute NC_GLOBAL geospatial_lon_min double -75.67819
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 5580.0
attribute NC_GLOBAL geospatial_vertical_min double 2.5
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://osprey.bco-dmo.org/dataset/985786 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
attribute NC_GLOBAL Northernmost_Northing double 42.83954
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 34.50102
attribute NC_GLOBAL summary String Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic matter (POM), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood.
 
A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed.  
 
This dataset includes the measurement of carbohydrate microarray (epitope) analyses of POM-derived carbohydrates from bulk waters collected in the western North Atlantic Ocean aboard R/V Endeavor (EN638) during May 2019. 
 
Note that the carbohydrate microarray data are only semiquantitative; while comparisons can be made for the abundance of a given epitope between stations and depths, the signal intensity cannot be used to compare signals of different epitopes, since the binding affinity for individual epitopes differs between antibodies.  Note also that analyses were performed for all sampled depths at all stations. There was, however,  no positive signal at depths that are shown as "0" in this dataset.
attribute NC_GLOBAL time_coverage_end String 2019-05-25T07:30:00Z
attribute NC_GLOBAL time_coverage_start String 2019-05-15T00:22:00Z
attribute NC_GLOBAL title String [Carb microarray analysis Atlantic 2019] - Carbohydrate microarray (epitope) analyses of POM-derived carbohydrates in the Western North Atlantic Ocean in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation)
attribute NC_GLOBAL Westernmost_Easting double -75.67819
variable deployment   String  
attribute deployment long_name String Deployment
attribute deployment units String unitless
variable station   String  
attribute station long_name String Station
attribute station units String unitless
variable latitude   float  
attribute latitude _CoordinateAxisType String Lat
attribute latitude actual_range float 34.50102, 42.83954
attribute latitude axis String Y
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   float  
attribute longitude _CoordinateAxisType String Lon
attribute longitude actual_range float -75.67819, -53.3949
attribute longitude axis String X
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable ISO_DateTime_Local   String  
attribute ISO_DateTime_Local long_name String Iso_datetime_local
attribute ISO_DateTime_Local units String unitless
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.55787972E9, 1.5587694E9
attribute time axis String T
attribute time ioos_category String Time
attribute time long_name String Iso_datetime_utc
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z
variable cast_number   String  
attribute cast_number long_name String Cast_number
attribute cast_number units String unitless
variable depth_sequence   String  
attribute depth_sequence long_name String Depth_sequence
attribute depth_sequence units String unitless
variable depth   float  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth actual_range float 2.5, 5580.0
attribute depth axis String Z
attribute depth ioos_category String Location
attribute depth long_name String Depth_actual
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable POM_L_filtered   float  
attribute POM_L_filtered actual_range float 5.0, 13.5
attribute POM_L_filtered long_name String Pom_l_filtered
attribute POM_L_filtered units String Liters
variable epitope   String  
attribute epitope long_name String Epitope
attribute epitope units String unitless
variable H2O   float  
attribute H2O actual_range float 0.0, 41.75
attribute H2O long_name String H2o
attribute H2O units String unitless
variable EDTA   float  
attribute EDTA actual_range float 0.0, 21.0
attribute EDTA long_name String Edta
attribute EDTA units String unitless
variable NaOH   float  
attribute NaOH actual_range float 0.0, 8.5
attribute NaOH long_name String Naoh
attribute NaOH units String unitless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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