ERDDAP > info > bcodmo_dataset_988643_v1

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[EN683 FLA-Bead Data and ULake] - Detection of polysaccharide enzymatic hydrolysis using a novel detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic (EN683 in May and June, 2022), and from a freshwater lake in North Carolina in 2025 (Substrate structural complexity and abundance control distinct mechanisms of microbially- driven carbon cycling in the ocean)		F   I   M	background			BCO-DMO	bcodmo_dataset_988643_v1

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	doi	String	10.26008/1912/bco-dmo.988643.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-53.8678
attribute	NC_GLOBAL	geospatial_lat_max	double	42.8701
attribute	NC_GLOBAL	geospatial_lat_min	double	33.74342
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-53.8678
attribute	NC_GLOBAL	geospatial_lon_min	double	-79.0935
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	4092.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	0.2
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://osprey.bco-dmo.org/dataset/988643
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	license	String	The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.
attribute	NC_GLOBAL	Northernmost_Northing	double	42.8701
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	33.74342
attribute	NC_GLOBAL	summary	String	A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). However, the current method is extremely work-intensive, and results are usually acquired months after initial sampling. Having a proxy to determine real-time enzymatic activity would be useful for scientists doing field work because it would provide a guide as to when and where optimal conditions are located to conduct water column sampling. In an effort to improve field detection techniques, a novel and relatively rapid detection method was developed to detect the hydrolysis of fluorescently-labeled laminarin that is linked to epoxy-activated-agarose beads. Laminarin was used because it is commonly found throughout the surface water of the ocean (Becker et al. 2020). Our goal is to detect potential enzymatic activity within 48 hours using minimal lab equipment while maintaining an accuracy similar to the current standard method used in previous research. Furthermore, we hope the method will provide real-time information for scientists in the field.    This dataset includes the detection of polysaccharide enzymatic hydrolysis using a novel and relatively rapid detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022, and from a local freshwater lake.
attribute	NC_GLOBAL	title	String	[EN683 FLA-Bead Data and ULake] - Detection of polysaccharide enzymatic hydrolysis using a novel detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic (EN683 in May and June, 2022), and from a freshwater lake in North Carolina in 2025 (Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean)
attribute	NC_GLOBAL	Westernmost_Easting	double	-79.0935
variable	deployment		String
attribute	deployment	long_name	String	Deployment
attribute	deployment	units	String	unitless
variable	station		String
attribute	station	long_name	String	Station
attribute	station	units	String	unitless
variable	latitude		float
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	actual_range	float	33.74342, 42.8701
attribute	latitude	axis	String	Y
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		float
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	actual_range	float	-79.0935, -53.8678
attribute	longitude	axis	String	X
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	sample_date		String
attribute	sample_date	long_name	String	Sample_date
attribute	sample_date	units	String	unitless
variable	sample_time		String
attribute	sample_time	long_name	String	Sample_time
attribute	sample_time	units	String	unitless
variable	ISO_DateTime_Local		String
attribute	ISO_DateTime_Local	long_name	String	Iso_datetime_local
attribute	ISO_DateTime_Local	units	String	unitless
variable	source		String
attribute	source	long_name	String	Source
attribute	source	units	String	unitless
variable	depth		float
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	actual_range	float	0.2, 4092.0
attribute	depth	axis	String	Z
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth_actual
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	temperature		float
attribute	temperature	actual_range	float	2.3, 27.0
attribute	temperature	long_name	String	Temperature
attribute	temperature	units	String	degrees Celsius
variable	salinity		float
attribute	salinity	actual_range	float	0.036, 36.68
attribute	salinity	long_name	String	Salinity
attribute	salinity	units	String	grams per liter (g/L)
variable	incubation_time		float
attribute	incubation_time	actual_range	float	0.0, 697.9
attribute	incubation_time	long_name	String	Incubation_time
attribute	incubation_time	units	String	hours
variable	live1		float
attribute	live1	actual_range	float	-2.95, 95.13
attribute	live1	long_name	String	Live1
attribute	live1	units	String	fluorescence units
variable	live2		float
attribute	live2	actual_range	float	-3.9, 98.99
attribute	live2	long_name	String	Live2
attribute	live2	units	String	fluorescence units
variable	live3		float
attribute	live3	actual_range	float	-2.58, 83.59
attribute	live3	long_name	String	Live3
attribute	live3	units	String	fluorescence units
variable	kill1		float
attribute	kill1	actual_range	float	-11.7, 52.2
attribute	kill1	long_name	String	Kill1
attribute	kill1	units	String	fluorescence units
variable	kill2		float
attribute	kill2	actual_range	float	-13.5, 45.0
attribute	kill2	long_name	String	Kill2
attribute	kill2	units	String	fluorescence units
variable	kill3		float
attribute	kill3	actual_range	float	-12.2, 50.0
attribute	kill3	long_name	String	Kill3
attribute	kill3	units	String	fluorescence units
variable	live_avg		float
attribute	live_avg	actual_range	float	-0.45, 78.07
attribute	live_avg	long_name	String	Live_avg
attribute	live_avg	units	String	fluorescence units
variable	live_avg_std		float
attribute	live_avg_std	actual_range	float	0.0, 26.12
attribute	live_avg_std	long_name	String	Live_avg_std
attribute	live_avg_std	units	String	fluorescence units
variable	kill_avg		float
attribute	kill_avg	actual_range	float	-12.37, 49.07
attribute	kill_avg	long_name	String	Kill_avg
attribute	kill_avg	units	String	fluorescence units
variable	kill_avg_std		float
attribute	kill_avg_std	actual_range	float	0.0, 4.19
attribute	kill_avg_std	long_name	String	Kill_avg_std
attribute	kill_avg_std	units	String	fluorescence units

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.